TETRAMERIC ALPHA/BETA HYDROLASE VARIANTS WITH INCREASED TEMPERATURE STABILITY AND METHODS OF USING AND PRODUCING THEREOF

• Main Authors: FRUHAUF, Sebastian, KRAINER, Florian, HOEBARTNER-GUSSL, Andreas, KILLINGER, Manuela, VOGTENTANZ, Gudrun
• Format: Patent
• Language: English; French
• Published: 14.04.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FODDER; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The present invention relates to alpha/beta hydrolase variants with improved properties compared to a parent alpha/beta hydrolase, e.g., having increased temperature stability, methods of using and producing such variants including methods of directing the quaternary structure formation from homo-dimers to homo-tetramers. La présente invention concerne des variants d'hydrolase alpha/bêta présentant des propriétés améliorées par rapport à une hydrolase alpha/bêta parente, par exemple, en présentant une stabilité accrue à la température, des procédés d'utilisation et de production de tels variants, y compris des procédés pour diriger la formation de la structure quaternaire pour passer des homo-dimères aux homo-tétramères.