METHOD FOR OBTAINING PLATELET-DERIVED MITOCHONDRIA AND USE THEREOF

• Main Authors: KANG, Young-cheol, HAN, Kyuboem, HA, Jong-Cheon
• Format: Patent
• Language: English; French; Korean
• Published: 17.03.2022
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TRAPPING; VINEGAR; WINE

The present invention relates to a method for obtaining active mitochondria by freeze storing platelets, and a use of the separated mitochondria and, more specifically, to a method for obtaining mitochondria by freezing and then thawing platelets and a pharmaceutical composition comprising as an active ingredient the mitochondria obtained by the method. The present invention provides a method for obtaining mitochondria by thawing platelets in a frozen state in a preservation solution, whereby mitochondria with activity stably maintained therein can be easily obtained and donated platelets which are discarded after cold storage within a short time can be provided with a commercial value. In addition, a pharmaceutical composition comprising platelet-derived mitochondria obtained by the method can be advantageously used for treating various diseases caused by mitochondrial dysfunction. La présente invention concerne un procédé d'obtention de mitochondries actives par congélation de plaquettes, et une utilisation des mitochondries séparées et, plus particulièrement, un procédé d'obtention de mitochondries par congélation puis décongélation des plaquettes et une composition pharmaceutique comprenant en tant que principe actif les mitochondries obtenues par le procédé. La présente invention concerne un procédé d'obtention de mitochondries par décongélation de plaquettes à l'état congelé dans une solution de conservation, ce qui permet d'obtenir facilement des mitochondries dont l'activité est maintenue de manière stable et de conférer une valeur commerciale aux plaquettes données qui sont rebutées après un court stockage au froid. De plus, une composition pharmaceutique comprenant des mitochondries dérivées de plaquettes obtenues par le procédé peut être avantageusement utilisée pour traiter diverses maladies causées par un dysfonctionnement mitochondrial. 본 발명은 혈소판을 냉동 보관하여 활성 미토콘드리아를 수득하는 방법과 분리된 미토콘드리아의 용도에 관한 것으로, 보다 상세하게는, 혈소판을 냉동 후 해동하여 미토콘드리아를 수득하는 방법 및 상기 방법에 의해 수득된 미토콘드리아를 유효성분으로 포함하는 약학 조성물에 관한 것이다. 본 발명은 보존용액에 냉동된 상태의 혈소판을 해동하여 미토콘드리아를 수득하는 방법을 제공함으로써 활성이 안정적으로 유지된 미토콘드리아를 손쉽게 얻을 수 있을 뿐만 아니라 짧은 시간 동안 냉장 보관 후 폐기되는 공여 혈소판에 대한 상업적 가치도 제공할 수 있다. 또한, 상기 방법에 의해 수득된 혈소판 유래 미토콘드리아를 포함하는 약학 조성물은 미토콘드리아 기능장애로 인해 유발되는 다양한 질환 치료에 유용하게 사용될 수 있다.