DONOR NUCLEIC ACID USED FOR GENE CORRECTION THROUGH MICROHOMOLOGY-MEDIATED END JOINING AND USES THEREOF

• Main Authors: DOAN, Thi Hai Duong, VU, Tien Van, KIM, Cha Young, LIM, Gah-Hyun, KIM, Jae Yean, SONG, Yeong Jong, SUNG, Yeon Woo, DAS, Swati, KIM, Jihae, JEONG, Jae Cheol, TRAN, Mil Thi
• Format: Patent
• Language: English; French; Korean
• Published: 13.01.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a donor nucleic acid used for gene correction through microhomology-mediated end joining and uses thereof. The use of the donor nucleic acid according to the present invention enables precise gene editing without limiting the location of a target gene and the number of bases. Therefore, the present invention is expected to be usefully used in the field of protein engineering through the introduction or removal of SNPs and the substitution of short amino acids, as well as in the new breeding development of crops. La présente invention concerne un acide nucléique donneur utilisé pour la correction génique par l'intermédiaire d'une jonction d'extrémités médiée par microhomologie et ses utilisations. L'utilisation de l'acide nucléique donneur selon la présente invention permet une édition de gène précise sans limiter l'emplacement d'un gène cible et le nombre de bases. Par conséquent, la présente invention est prévue pour être utilement utilisée dans le domaine de l'ingénierie des protéines par l'introduction ou l'élimination de SNP et la substitution d'acides aminés courts, ainsi que dans la nouvelle amélioration génétique de cultures. 본 발명은 미세상동성 기반 말단 결합을 통한 유전자 교정에 이용되는 공여자 핵산 및 이의 용도에 관한 것으로, 본 발명에 따른 공여자 핵산을 이용하면 표적 유전자의 위치 및 염기 수에 제한 없이 정밀한 유전자 편집이 가능하므로, 작물의 신육종 개발 뿐만 아니라 SNP의 도입이나 제거 및 짧은 아미노산 치환을 통한 단백질 엔지니어링 분야에 유용하게 사용할 수 있을 것으로 기대된다.