METHODS FOR SITE SPECIFIC CONJUGATION OF PROTEINS CONTAINING GLYCOSYLATED FC DOMAINS

Provided herein are methods for site-specific conjugation of glycan intact antibodies by a transglutaminase. According to particular embodiments, the reaction conditions are maintained or reduced to a low-ionic strength condition, which allows for efficient and fast conjugation without the need for...

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Bibliographic Details
Main Authors MCCAULEY, Michael J, DUDKIN, Vadim, GOLDBERG, Shalom, WILEY, Kristen
Format Patent
LanguageEnglish
French
Published 25.11.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Provided herein are methods for site-specific conjugation of glycan intact antibodies by a transglutaminase. According to particular embodiments, the reaction conditions are maintained or reduced to a low-ionic strength condition, which allows for efficient and fast conjugation without the need for antibody deglycosylation. Also described are pharmaceutical compositions and uses related to the conjugation method. La présente invention concerne des procédés de conjugaison régiospécifique d'anticorps intacts de glycane par une transglutaminase. Selon des modes de réalisation particuliers, les conditions de réaction sont maintenues ou réduites à une condition de faible force ionique, ce qui permet une conjugaison efficace et rapide sans avoir besoin d'une déglycosylation d'anticorps. L'invention concerne également des compositions pharmaceutiques et des utilisations associées au procédé de conjugaison.
Bibliography:Application Number: WO2021IB54310