LACHNOSPIRACEAE SP. CAS12A MUTANTS WITH ENHANCED CLEAVAGE ACTIVITY AT NON-CANONICAL TTTT PROTOSPACER ADJACENT MOTIFS

Described herein are CAS12A mutants from Lachnospiraceae bacterium and methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme. L'invention concerne des mutants de CAS12A à partir de La...

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Bibliographic Details
Main Authors VAKULSKAS, Christopher Anthony, ZHANG, Liyang
Format Patent
LanguageEnglish
French
Published 04.11.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Described herein are CAS12A mutants from Lachnospiraceae bacterium and methods for use thereof. These mutants have enhanced DNA cleavage activities at non-canonical TTTT protospacer adjacent motifs (PAM) compared to the wild-type enzyme. L'invention concerne des mutants de CAS12A à partir de Lachnospiraceae bacterium, ainsi que des procédés d'utilisation de ceux-ci. Ces mutants présentent des activités améliiorées de clivage d'ADN au niveau de motifs adjacents de protoespaceur TTTT (PAM) non canonique par rapport à l'enzyme de type sauvage.
Bibliography:Application Number: WO2021US30089