PREPARATION OF HUMAN BASIC FIBROBLAST GROWTH FACTOR BY USING BACILLUS SUBTILIS AND ENDONUCLEASE

• Main Authors: KWONG, Wai Yeung, CHUNG, Shu Kun Christopher
• Format: Patent
• Language: Chinese; English; French
• Published: 16.09.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided is a nucleic acid construct, which comprises an insert, and the insert comprises, from the 5' end to the 3' end, a a polynucleotide sequence that encodes a short peptide affinity tag, a trans-splicing intein derived from Anabaena, and an exogenous polypeptide; and the short peptide affinity tag serves as an N-terminal extein of the trans-splicing intein, and the exogenous polypeptide serves as a C-terminal extein of the trans-splicing intein. Further provided are an expression vector and a host cell that comprise the construct and a method for producing and purifying foreign proteins by using said expression vector and host cell. L'invention concerne une construction d'acide nucléique, qui comprend un insert, et l'insert comprend, de l'extrémité 5' à l'extrémité 3', une séquence polynucléotidique qui code pour un marqueur d'affinité peptidique court, une intéine de trans-épissage dérivée d'Anabaena, et un polypeptide exogène ; et le marqueur d'affinité peptidique court sert d'extéine N-terminale de l