METHOD FOR ISOLATING AUTOLOGOUS TUMOR ANTIGEN-REACTIVE CD8 T CELL BY USING CD71, AND APPLICATION THEREOF

• Main Authors: LEE, Eun Sook, CHOI, Beom Kyu, KIM, Seon Hee
• Format: Patent
• Language: English; French; Korean
• Published: 12.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention relates to: a method for isolating an autologous tumor antigen-reactive CD8 T cell by using CD71; and an application thereof. When an autologous tumor antigen-reactive CD8 T cell is isolated using an isolation method according to the present invention, CD8 T cells that react to various tumor antigens are also isolated in addition to the CD8 T cell specific for autologous tumor antigens, and thus the number of CD8 T cells can be amplified in large quantities to provide a significant antitumor effect, even with lower quantities of blood. Therefore, the method can be effectively used for immunotherapy, etc., in which a temporary immunodeficiency is induced in order to perform a treatment such as adoptive cell treatment. La présente invention concerne : un procédé d'isolement d'un lymphocyte T CD8 réactif à l'antigène tumoral autologue à l'aide de CD71 ; et une application de celui-ci. Lorsqu'un lymphocyte T CD8 réactif à un antigène tumoral autologue est isolé à l'aide d'un procédé d'isolement selon la présente invention, des lymphocytes T CD8 qui réagissent à divers antigènes tumoraux sont également isolés en plus du lymphocyte T CD8 spécifique des antigènes tumoraux autologues, et ainsi le nombre de lymphocytes T CD8 peut être amplifié en grandes quantités pour fournir un effet antitumoral significatif, même avec des quantités moindres de sang. Par conséquent, le procédé peut être efficacement utilisé pour l'immunothérapie, etc, dans laquelle une immunodéficience temporaire est induite afin d'effectuer un traitement tel qu'un traitement cellulaire adoptif. 본 발명은 CD71을 이용하여 자가암항원 반응성 CD8 T 세포를 분리하는 방법 및 이의 응용에 대한 것이다. 본 발명의 분리방법을 이용하여 자가암항원 반응성 CD8 T 세포를 분리하는 경우 자가암항원에 특이적인 CD8 T 세포 이외에 다양한 암항원에 반응하는 CD8 T 세포도 함께 분리되므로, 보다 적은 혈액으로도 대량으로 CD8 T 세포수를 증폭시켜 유의적인 항암효과를 제공할 수 있다. 따라서 입양 세포 치료 등 치료를 위해 일시적인 면역결핍을 유도하는 면역치료 등에 효과적으로 사용될 수 있다.