KIT, SYSTEM, AND FLOW CELL

• Main Authors: KHURANA, Tarun Kumar, DAGGUMATI, Pallavi, WU, Yir-Shyuan, CHEN, Tyler
• Format: Patent
• Language: English; French
• Published: 05.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

An example of a kit includes a library preparation fluid, a sample fluid, and an enrichment fluid. The library preparation fluid includes library preparation beads, where each library preparation bead includes a first solid support, and a transposome attached to the first solid support. The fluid includes a genomic deoxyribonucleic acid sequence. The enrichment fluid includes target capture beads, where each target capture bead includes a second solid support, and capture probes attached to the second solid support. Each of the capture probes includes a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid in the sample fluid. Un kit donné à titre d'exemple comprend un fluide de préparation de bibliothèque, un fluide d'échantillon et un fluide d'enrichissement. Le fluide de préparation de bibliothèque comprend des billes de préparation de bibliothèque, chaque bille de préparation de bibliothèque comprenant un premier support solide et un transposome fixé au premier support solide. Le fluide comprend une séquence d'acide désoxyribonucléique génomique. Le fluide d'enrichissement comprend des billes de capture cibles, chaque bille de capture cible comprenant un second support solide, et des sondes de capture fixées au second support solide. Chacune des sondes de capture comprend une séquence d'acide désoxyribonucléique monocaténaire qui est complémentaire d'une région ciblée de l'acide désoxyribonucléique génomique dans le fluide d'échantillon.