FERMENTATION PATHWAY FOR PRODUCING MALONIC ACID

• Main Authors: RUSH, Brian Jeffrey, KOHLI, Nandita, JAUERT, Peter Alan, BRADY, Keith, KOZLOWICZ, Briana Kristen, MATTHIJS, Michiel, LOUIE, Michael Tai Man, WOLTER, Travis Robert, PARIMI, Naga Sirisha, HASS, Cathy Staloch, POOR, Catherine Bradshaw, LIAO, Hans H
• Format: Patent
• Language: English; French
• Published: 01.07.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present disclosure provides an engineered microorganism capable of producing malonic acid, malonate, esters of malonic acid, or mixtures thereof. The engineered microorganism includes a malonate-semialdehyde dehydrogenase that is heterologous to a native form of the engineered microorganism and comprises at least 90% sequence identity to any one of SEQ ID Nos: 7, 9, 11, 13, 15, 17, 19, 21, 23, 27, 29, and 31, wherein the engineered microorganism is capable of producing about 9 g/L to about 250 g/L of malonic acid, malonate, esters of malonic acid, or mixtures thereof. La présente invention concerne un micro-organisme modifié capable de produire de l'acide malonique, du malonate, des esters d'acide malonique ou des mélanges de ceux-ci. Le micro-organisme modifié comprend une malonate-semialdéhyde déshydrogénase qui est hétérologue à une forme native du micro-organisme modifié et comprend au moins 90 % d'identité de séquence avec l'une quelconque des SEQ ID NO : 7, 9, 11, 13, 15, 17, 19, 21, 23, 27, 29 et 31, le micro-organisme modifié étant capable de produire d'environ 9 g/L à environ 250 g/L d'acide malonique, de malonate, d'esters d'acide malonique ou de mélanges de ceux-ci.