METHOD FOR PRODUCING SEQUENCING LIBRARY

• Main Authors: OTSUBO, Yuki, MATSUMURA, Shoji
• Format: Patent
• Language: English; French; Japanese
• Published: 20.05.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The purpose of the invention is to provide a sequencing library that gives reduced sequencing error. Provide is a method for producing a sequencing library comprises fragmenting a sample DNA; and treating the prepared sample DNA fragments with a single-strand specific nuclease to remove single-strand moieties from the fragments. L'objectif de l'invention est de fournir une bibliothèque de séquençage qui donne une erreur de séquençage réduite. L'invention concerne un procédé de production d'une bibliothèque de séquençage qui comprend la fragmentation d'un échantillon d'ADN ; et le traitement des fragments d'ADN d'échantillon préparés avec une nucléase spécifique à un seul brin pour éliminer des fractions à un seul brin des fragments. シーケンシングエラーを低減させるシーケンシング用ライブラリの提供。サンプルＤＮＡを断片化すること；及び、調製したサンプルＤＮＡの断片を１本鎖特異的ヌクレアーゼで処理し、該断片から１本鎖部分を除去すること、を含むシーケンシング用ライブラリの調製方法。