CELL CULTURE METHOD, METHOD FOR PRODUCING CULTURE SOLUTION, CULTURE SOLUTION AND CULTURE DEVICE

• Main Authors: YAMADA Akitoshi, YAMAMOTO Akira, ISHINAGA Hiroyuki, OZAKI Teruo, YANAI Yumi, KASHINO Toshio, KUBOTA Masahiko, IMANAKA Yoshiyuki
• Format: Patent
• Language: English; French; Japanese
• Published: 06.05.2021
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The purpose of the present invention is to efficiently produce three-dimensional cells in a large amount. For this purpose, cells 603 are cultured using a culture solution 602 containing ultrafine bubbles 11 that are generated by heating a heating element 10 and thus causing film boiling at the interface of a liquid W and the heating element. Le but de la présente invention est de produire efficacement des cellules tridimensionnelles en grande quantité. À cet effet, des cellules (603) sont cultivées à l'aide d'une solution de culture (602) contenant des bulles ultrafines (11) qui sont générées par chauffage d'un élément chauffant (10) et provoquant ainsi l'ébullition du film à l'interface d'un liquide (W) et de l'élément chauffant. ３次元細胞を効率的に且つ多量に生産する。そのために、発熱素子１０を発熱させて液体Ｗと発熱素子の界面に膜沸騰を生じさせることにより生成されたウルトラファインバブル１１を含む培養液６０２を用い、細胞６０３を培養する