HPV DETECTION KIT AND HPV DETECTION METHOD USING SAME

• Main Authors: JANG, Siun, PARK, Young Suk, LEE, Chanhyo, HWANG, Byoungoh
• Format: Patent
• Language: English; French; Korean
• Published: 29.10.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a Human Papillomavirus (HPV) detection kit and an HPV detection method using same, and, particularly, relates to: a kit for detecting HPV, comprising (a) a primer, which comprises a binding site complementary binding to an HPV-specific gene and a nucleic acid oligomer that is non-complementary to an HPV-specific gene and (b) a probe capable of identifying the amplified product; and an HPV detection method and an HPV infection diagnosis method which use same. The present invention can diagnose HPV infection by using only a small amount of target genes, and amplifies and diagnoses a high-risk genotype HPV-specific gene, and thus can rapidly and accurately enable the qualitative and molecular diagnosis of HPV. La présente invention concerne un kit de détection de papillomavirus humain (VPH) ainsi qu'un procédé de détection de VPH l'utilisant et, en particulier, (a) une amorce qui comprend une liaison complémentaire de site de liaison à un gène spécifique du VPH et un oligomère d'acide nucléique qui n'est pas complémentaire d'un gène spécifique du VPH et (b) une sonde apte à identifier le produit amplifié ; et un procédé de détection de VPH et un procédé de diagnostic d'infection par le VPH qui utilisent ce dernier. La présente invention peut diagnostiquer une infection par le VPH en utilisant uniquement une petite quantité de gènes cibles et amplifie et diagnostique un gène spécifique du VPH de génotype à haut risque et peut ainsi permettre le diagnostic qualitatif et moléculaire du VPH rapidement et avec précision. 본 발명은 HPV 검출용 키트 및 이를 이용한 HPV 검출방법에 관한 것으로, 구체적으로 (a) HPV (Human Papillomavirus) 특이적 유전자와 결합하는 상보적 결합 부위 및 HPV 특이적 유전자와 비상보적인 핵산 올리고머를 함유하는 프라이머, 및 (b) 상기 증폭된 산물을 확인할 수 있는 프로브를 포함하는 HPV를 검출하기 위한 키트, 이를 이용하여 HPV를 검출하는 방법 및 HPV 감염 여부의 진단방법에 관한 것이다. 본 발명에 따르면, 미량의 검체 유전자만으로 HPV 감염 여부의 진단이 가능하도록 하고, 고위험 유전형 HPV 특이적 유전자를 증폭하여 진단함으로써, HPV에 대한 신속 정확한 정성적 분자진단이 가능하도록 한다.