PHOSPHOLIPASE D MUTANT, USE THEREOF AND METHOD FOR PREPARING PHOSPHATIDYLSERINE BY MEANS OF SAME

Disclosed are a phospholipase D mutant, a use thereof and a method for preparing phosphatidylserine by means of same, which relate to the technical field of bio-enzyme catalysis. Provided is a phospholipase D mutant with an enzyme activity inclined more towards a phosphatidyl transfer activity. Comp...

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Main Authors FU, Rongzhao, TANG, Xiaoxin, ZHU, Zhiqiang, LIU, Wenshan, LI, Zhenwei
Format Patent
LanguageChinese
English
French
Published 02.07.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Disclosed are a phospholipase D mutant, a use thereof and a method for preparing phosphatidylserine by means of same, which relate to the technical field of bio-enzyme catalysis. Provided is a phospholipase D mutant with an enzyme activity inclined more towards a phosphatidyl transfer activity. Compared with the amino acid sequence of wild-type phospholipase D, the phospholipase D mutant has a mutation at at least one position selected from positions 93, 105, 215, 216, 228 and 486. L'invention concerne un mutant de phospholipase D, son utilisation et un procédé de préparation de phosphatidylsérine au moyen de celui-ci, ses éléments étant liés au domaine technique de la catalyse bioenzymatique. L'invention concerne un mutant de phospholipase D ayant une activité enzymatique tendant plus vers une activité de transfert de phosphatidyle. Par comparaison avec la séquence d'acides aminés de la phospholipase D de Type sauvage, le mutant de phospholipase D présente une mutation en au moins une position choisie parmi
Bibliography:Application Number: WO2018CN125222