APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING

The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro. The disclosure further describes modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adj...

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Bibliographic Details
Main Authors CHAIKIND, Brian, VAN ROSSUM, Hendrik M, PERKOVICH, Paul, MILLER, Aaron, PATEL, Kedar, SZYJKA, Shawn
Format Patent
LanguageEnglish
French
Published 17.09.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The disclosure describes methods, compositions, and kits for high throughput DNA assembly reactions in vitro. The disclosure further describes modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations. High throughput methods of CRISPRi and CRISPRa are also disclosed. L'invention concerne des procédés, des compositions, et des kits pour des réactionsd'assemblage d'ADN à haut rendement in vitro. L'invention concerne en outre des constructions d'ADN CRISPR modulaires comprenant des parties d'ADN d'inserts modulaires flanquées de segments d'étiquettes de clonage comprenant des combinaisons de séquences de motif adjacent protoespaceur/protoespaceur CRISPR pré-validées. L'invention concerne également des procédés de CRISPRi et CRISPRa à haut rendement.
Bibliography:Application Number: WO2019US46555