RECOMBINANT VECTOR FOR PRODUCING CARDIAC HYPERTROPHY ANIMAL MODEL, CARDIAC HYPERTROPHY ANIMAL MODEL TRANSFORMED WITH RECOMBINANT VECTOR, AND PRODUCTION METHOD THEREFOR

• Main Authors: SEOK, Hee Young, CHI, Sung Wook
• Format: Patent
• Language: English; French; Korean
• Published: 30.04.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

The present invention relates to a recombinant vector for producing a cardiac hypertrophy animal model, a cardiac hypertrophy animal model transformed with the recombinant vector, and a production method therefor. When a recombinant vector according to the present invention is specifically expressed in mouse cardiomyocytes, only non-canonical G:A wobble base sequence target sites of miRNA are recognized and inhibited, so that a biological function of increasing the size of cardiomyocytes is exhibited so as to induce cardiac hypertrophy, and thus an animal model in which cardiac hypertrophy has been induced is expected to be produced so as to be effectively used for studying the progression, pathology and physiological mechanism of heart diseases including cardiac hypertrophy at the individual level. L'invention concerne un vecteur recombiné pour produire un modèle animal d'hypertrophie cardiaque, un modèle animal d'hypertrophie cardiaque transformé avec le vecteur recombiné, et un procédé de production correspondant. Lorsqu'un vecteur recombiné selon la présente invention est spécifiquement exprimé dans des cardiomyocytes de souris, seul des sites cibles de séquence de bases d'appariement bancal non canonique G:A du miARN sont reconnus et inhibés, de telle sorte qu'une fonction biologique d'augmentation de la taille de cardiomyocytes est présentée de façon à induire une hypertrophie cardiaque, et par conséquent, un modèle animal présentant une hypertrophie cardiaque induite est censé être produit de façon à être efficacement utilisé pour étudier la progression, la pathologie et le mécanisme physiologique de maladies cardiaques comprenant l'hypertrophie cardiaque au niveau individuel. 본 발명은 심비대증 동물 모델 제작용 재조합 벡터, 상기 재조합 벡터로 형질전환된 심비대증 동물 모델 및 이의 제작 방법에 관한 것으로, 본 발명에 따른 재조합 벡터를 마우스의 심근 세포에 특이적으로 발현시킬 경우, 마이크로RNA의 비정규 G:A 워블 염기 배열 표적 사이트만을 인식하여 억제함으로써 심근 세포의 크기가 증가하는 생물학적 기능이 나타나고, 이로 인해 심비대증이 유도되는바, 심비대증이 유도된 동물 모델을 제조하여 개체 수준에 있어서 심비대증을 포함한 심장 질환의 진행 경과 및 병리, 생리학적 기전 연구에 유용하게 이용할 수 있을 것으로 기대된다.