MONOCLONAL ANTIBODY N16-F2 SPECIFIC TO HUMAN NAIVE PLURIPOTENT STEM CELLS

• Main Authors: SEO, Se Ri, RYU, Chun Jeih, CHOI, Hong Seo
• Format: Patent
• Language: English; French; Korean
• Published: 30.04.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to: a monoclonal antibody specifically binding to human naive pluripotent stem cells; and a hybridoma producing same and, particularly to: monoclonal antibody N16-F2, which binds to human naive pluripotent stem cells but not to human primed pluripotent stem cells or has significantly weak binding capacity; hybridoma N16-F2 for producing the monoclonal antibody; an assay kit comprising monoclonal antibody N16-F2; and a composition for removing human pluripotent stem cells. La présente invention concerne : un anticorps monoclonal se liant de manière spécifique à des cellules souches pluripotentes naïves humaines ; et un hybridome produisant celui-ci ; l'invention concerne en particulier : un anticorps monoclonal N16-F2, qui se lie à des cellules souches pluripotentes naïves humaines mais pas à des cellules souches pluripotentes sensibilisées humaines ou qui a une capacité de liaison significativement faible ; un hybridome N16-F2 permettant de produire l'anticorps monoclonal ; un kit de dosage comprenant l'anticorps monoclonal N16-F2 ; et une composition permettant d'éliminer les cellules souches pluripotentes humaines. 본 발명은 인간 순수 만능줄기세포 (human naive pluripotent stem cell)에 특이적으로 결합하는 단일클론항체 및 이를 생산하는 하이브리도마에 관한 것으로서, 구체적으로는 인간 순수만능줄기세포에는 결합하고 인간 준 만능줄기세포에는 결합하지 않거나 결합력이 현저히 약한 단일클론항체 N16-F2, 상기 단일클론항체를 생산하는 하이브리도마 N16-F2, 상기 단일클론항체 N16-F2를 포함하는 검정키트 및 인간 만능줄기세포 제거용 조성물에 관한 것이다.