METHOD FOR PREPARING AND CRYOPRESERVING CANCER ANTIGEN-SPECIFIC CD8+ T CELLS

• Main Authors: KIM, Young Ho, KIM, Mun Ki, KANG, You Hyun, KIM, Kwang Hee, LEE, Sae Rom, KWON, Byoung Se
• Format: Patent
• Language: English; French; Korean
• Published: 13.02.2020
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PEST REPELLANTS OR ATTRACTANTS; PHYSICS; PLANT GROWTH REGULATORS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; TRAPPING; VINEGAR; WINE

The present invention relates to a method for preparing cancer antigen-specific CD8 + T cells, comprising the steps of: (a) activating CD8 + T cells and expressing a co-stimulatory factor of the CD8 + T cells in PBMCs comprising cancer antigen-specific CD8 + T cells; (b) selecting, from among the cells activated in step (a), CD8 + T cells in which a co-stimulatory factor is expressed; (c) increasing the number of the CD8 + T cells selected in step (b); (d) freezing the CD8 + T cells obtained in step (c); and (e) thawing the CD8 + T cells frozen in step (d). La présente invention concerne un procédé de préparation de cellules T CD8 + spécifiques d'un antigène cancéreux, comprenant les étapes suivantes consistant : (a) à activer des cellules T CD8 + et à exprimer un facteur co-stimulateur des cellules T CD8 + dans des PBMC comprenant des cellules T CD8 + spécifiques d'un antigène cancéreux; (b) à sélectionner, parmi les cellules activées dans l'étape (a), de cellules T CD8 + dans lesquelles un facteur co-stimulateur est exprimé; (c) à augmenter le nombre de cellules T CD8 + sélectionnées dans l'étape (b); (d) à congeler les cellules T CD8 + obtenues dans l'étape (c); et (e) à décongeler les cellules T CD8 + congelées dans l'étape (d). 본 발명은 (a) 암항원 특이적 CD8 + T 세포가 포함된 PBMC 중 상기 CD8 + T 세포를 활성화시켜 상기 CD8 + T 세포의 공동 자극 인자(co-stimulatory factor)를 발현시키는 단계; (b) 상기 (a) 단계에서 활성화된 세포들 중 공동 자극 인자가 발현된 CD8 + T 세포를 선별하는 단계; (c) 상기 (b) 단계에서 선별된 CD8 + T 세포의 수를 증가시키는 단계; (d) 상기 (c) 단계에서 수득된 CD8 + T 세포를 동결시키는 단계; 및 (e) 상기 (d) 단계에서 동결된 CD8 + T 세포를 해동하는 단계를 포함하는, 암항원 특이적 CD8 + T 세포 제조 방법에 관한 것이다.