CHROMATOGRAPHIC MATRIX, METHOD AND USES THEREOF

• Main Authors: PEREIRA DE SOUSA, Fani, CLAUDINO MARTINS, João Carlos, FREIRE MARTINS, Mara Guadalupe, DE SAMPAIO RODRIGUES QUEIROZ, João António, CARVALHO NEVES, Márcia, NUNES PEREIRA, Patrícia Alexandra, QUARESMA HENRIQUES PEDRO, Augusto
• Format: Patent
• Language: English; French; Portuguese
• Published: 19.09.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SEPARATION; SPIRITS; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

The present disclosure concerns the development of a novel stationary phase for the separation of nucleic acids in the context of preparative chromatography, using a chromatographic matrix, preferably a macroporous matrix, particularly an epoxylated methacrylic polymer matrix modified with the immobilized ionic liquid (IL) 1-methylimidazolium chloride as a multimodal ligand. La présente invention concerne la mise au point d'une nouvelle phase stationnaire pour la séparation d'acides nucléiques dans le cadre d'une chromatographie préparative, au moyen d'une matrice chromatographique, de préférence une matrice macroporeuse, notamment une matrice d'un polymère méthacrylique époxylé, modifiée avec le liquide ionique (LI) chlorure de 1-méthylimidazole immobilisé en tant que ligand multimodal. A presente divulgação diz respeito ao desenvolvimento de uma nova fase estacionária para a separação de ácidos nucleicos no contexto de cromatografia preparativa, utilizando uma matriz cromatográfica, de preferência uma matriz macroporosa, em particular uma matriz de um polímero metacrílico epoxilado, modificada com o líquido iónico (LI) cloreto de 1-metilimidazólio imobilizado como ligando multimodal.