MULTIMERIC PROTEIN DISPLAY SYSTEM UTILIZING CELL MEMBRANE FLUIDITY

• Main Authors: YOON, Hyun Woung, JO, Migyeong, HWANG, Bora, JUNG, Sang Taek
• Format: Patent
• Language: English; French; Korean
• Published: 25.04.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a display system of a multimeric target protein utilizing cell membrane fluidity. A display system of the present invention has remarkably enhanced convenience (simple medium preparation, reduced incubation time for screening, employment of single promoters, etc.) compared to that of conventional full-length IgG display systems or Fc display systems using PelB and exhibits a smaller % CV value and a larger difference in fluorescence signal intensity between negative and positive clones than conventional full-length IgG display techniques, thus being more effective for screening using a target protein display. La présente invention concerne un système d'affichage d'une protéine cible multimère utilisant la fluidité de la membrane cellulaire. Un système d'affichage de la présente invention présente un niveau pratique remarquablement amélioré (préparation simple du milieu, temps d'incubation réduit pour le criblage, utilisation de promoteurs uniques, etc.) comparé à celui de systèmes d'affichage IgG de pleine longueur classique ou de systèmes d'affichage Fc utilisant PelB et faisant preuve d'une plus petite valeur de % CV et d'une plus grande différence dans l'intensité du signal de fluorescence entre les clones négatifs et positifs que les techniques d'affichage d'IgG de pleine longueur classique, devenant ainsi plus efficace pour le criblage en utilisant un affichage à protéine cible. 본 발명은 세포막의 유동성을 이용한 다중체인 목적단백질의 디스플레이 시스템에 관한 것이다. 본 발명의 디스플레이 시스템은 기존의 전장 IgG 디스플레이 시스템이나 PelB를 이용한 Fc 디스플레이 시스템에 비해 월등히 높아진 편의성(간단한 배지의 제조, 스크리닝을 위한 배양시간 감소, 단일 프로모터의 사용 등)을 가지며, 기존의 전장 IgG 디스플레이 기법보다 %CV 값이 작고 음성 클론과 양성 클론 간 형광 신호 값의 차이가 커 목적단백질 디스플레이를 이용한 스크리닝에 더욱 효율적이다.