METHOD FOR MODULATING GENE EXPRESSION IN PLANTS, VECTORS, USE OF THE VECTORS AND USE OF PROMOTERS OF THE GENES SCDHN, SCLNC AND SCTIP2-3

• Main Authors: TEIXEIRA, Marcelo Menossi, TSUNADA, Max Seiji, ALESSIO, Valter Miotto, POLETTO, Isabella De Oliveira Conte
• Format: Patent
• Language: English; French; Portuguese
• Published: 11.04.2019
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WATERING; WINE

The present invention relates to a method for modulating gene expression in sugarcane through use of promoters isolated from genes with an expression profile induced by water deficit in leaves, stems and roots, or preferably in sugarcane roots. Furthermore, the present invention relates to the use of three nucleotide sequences which act as promoters in the regulation of genes encoding a dehydrin (ScDHN), a long non-coding transcript (ScLNC), and a tonoplast intrinsic protein 2-3 (ScTIP2-3), for activating genes of interest in transgenic plants for the purpose of increasing productivity and tolerance to abiotic stress. La présente invention concerne une méthode de modulation de l'expression génique dans la canne à sucre par utilisation de promoteurs isolés de gènes à profil d'expression induites par déficit hydrique dans les feuilles, les tiges et les racines ou de préférence dans les racines de la canne à sucre. En outre, la présente invention concerne l'utilisation de trois séquences de nucléotides qui agissent comme promoteurs de la régulation des gènes codant pour une déidrine (ScDHN), un transcrit long non codant (ScLNC) et une protéine intrinsèque de tonoplaste 2-3 (ScTIP2-3) pour activation de gènes d'intérêt dans des plantes transgéniques en vue d'augmenter la productivité et la tolérance au stress abiotique. A presente invenção refere-se a um método de modulação da expressão gênica em cana-de-açúcar através do uso de promotores isolados de genes com perfil de expressão induzida por déficit hídrico em folhas, colmo e raízes ou preferencialmente em raízes de cana-de-açúcar. Adicionalmente, a presente invenção refere-se ao uso de três sequências de nucleotídeos que atuam como promotores da regulação dos genes que codificam uma deidrina (ScDHN), um transcrito longo não codificante (ScLNC), e uma proteína intrínseca de tonoplasto 2-3 (ScTIP2-3) para ativação de genes de interesse em plantas transgências com o objetivo de aumentar produtividade e tolerância ao estresse abiótico.