EXTRACTION DEVICE, EXTRACTION METHOD AND EXTRACTION SYSTEM FOR EXTRACTING TARGET SUBSTANCE

• Main Authors: NA, Won-Hwi, LEE, Ho-Yoon, SHIN, Se-Hyun, SEO, Chang-Deok
• Format: Patent
• Language: English; French; Korean
• Published: 06.06.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRANSPORTING; VINEGAR; WINE

The present invention relates to an extraction device, an extraction method and an extraction system for extracting a target substance. The extraction device according to the present invention comprises: a sample kit; a porous membrane installed in the sample kit and having a target substance bound thereto; an elution buffer which is stacked over the porous membrane in the sample kit, passes through the porous membrane and thereby elutes the target substance out of the porous membrane; and a bipolar solution which has a different polarity from the elution buffer and is therefore not mixed therewith and is stacked over the elution buffer in the sample kit, wherein the elution buffer and the bipolar solution pass through the porous membrane in order, and, when passing through the porous membrane, the bipolar solution pushes out the elution buffer left in the porous membrane. Accordingly, a target substance such as a nucleic acid can be extracted even without using a centrifugation method or applying high-speed centrifugation. La présente invention concerne un dispositif d'extraction, un procédé d'extraction et un système d'extraction pour extraire une substance cible. Le dispositif d'extraction selon la présente invention comprend : un kit d'échantillon; une membrane poreuse installée dans le kit d'échantillon et ayant une substance cible liée à celle-ci; un tampon d'élution qui est empilé sur la membrane poreuse dans le kit d'échantillon, passe à travers la membrane poreuse et élue ainsi la substance cible hors de la membrane poreuse; et une solution bipolaire qui a une polarité différente du tampon d'élution et, par conséquent, n'est pas mélangée à celui-ci et est empilée sur le tampon d'élution dans le kit d'échantillon, le tampon d'élution et la solution bipolaire passant à travers la membrane poreuse dans l'ordre et, lors du passage à travers la membrane poreuse, la solution bipolaire poussant le tampon d'élution restant dans la membrane poreuse. Par conséquent, une substance cible telle qu'un acide nucléique peut être extraite, même sans utiliser de procédé de centrifugation ou appliquer de centrifugation à grande vitesse. 본 발명은 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템에 관한 것이다. 본 발명에 따른 추출 장치는 시료 키트와, 상기 시료 키트의 내부에 설치되어 상기 타겟 물질이 바인딩되는 다공막과, 상기 시료 키트 내에서 상기 다공막에 적층되어 상기 다공막을 통과하면서 상기 다공막으로부터 상기 타겟 물질을 용출하는 용출 용액과, 상기 용출 용액과 상이한 극성을 가져 상기 용출 용액과 섞이지 않는 상태로 상기 시료 키트 내에서 상기 용출 용액에 적층되는 이극성 용액을 포함하며; 상기 용출 용액과 상기 이극성 용액이 순차적으로 상기 다공막을 통과하고; 상기 이극성 용액이 상기 다공막을 통과할 때 상기 다공막에 잔존하는 상기 용출 용액을 밀어내는 것을 특징으로 한다. 이에 따라, 원심 분리 방식을 이용하지 않거나 고속의 원심 분리를 적용하지 않으면서도 핵산과 같은 타겟 물질을 추출할 수 있다.