SYSTEMS AND METHODS FOR TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF USING INTEGRATED PRIMING SITES

Disclosed herein are genome editing systems and related methods which allow for the detection and quantitative measurement of all possible on-target gene editing outcomes, including targeted integration. The compositions and methods described herein rely on the use of donor templates comprising a 5&...

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Bibliographic Details
Main Authors MARGULIES, Carrie, COTTA-RAMUSINO, Cecilia
Format Patent
LanguageEnglish
French
Published 17.01.2019
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Disclosed herein are genome editing systems and related methods which allow for the detection and quantitative measurement of all possible on-target gene editing outcomes, including targeted integration. The compositions and methods described herein rely on the use of donor templates comprising a 5' homology arm, a cargo, a one or more priming sites, a 3' homology arm, and optionally stuffer sequence. L'invention concerne des systèmes d'édition du génome et des procédés associés qui permettent la détection et la mesure quantitative de tous les résultats possibles d'édition de gènes sur cible, y compris l'intégration ciblée. Les compositions et les procédés selon l'invention reposent sur l'utilisation de modèles de donneurs comprenant un bras d'homologie en 5', un chargement, un ou plusieurs sites d'amorçage, un bras d'homologie en 3' et éventuellement une séquence de remplissage.
Bibliography:Application Number: WO2018US42040