MULTIPLEX FLUOROIMMUNOASSAY METHOD FOR RAPIDLY DISTINGUISHING RABBIT HEMORRHAGIC DISEASE VIRUS, SENDAI VIRUS AND LAPINE ROTAVIRUS, AND REAGENT

• Main Authors: GUO, Pengju, ZHANG, Yu, CONG, Feng, HUANG, Ren, WU, Miaoli, CHEN, Meili
• Format: Patent
• Language: Chinese; English; French
• Published: 27.12.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Disclosed are a multiplex fluoroimmunoassay method for rapidly distinguishing the rabbit hemorrhagic disease virus, Sendai virus and lapine rotavirus, and a reagent. The fluoroimmunoassay method comprises: obtaining a target amplification fragment by means of PCR; then hybridizing an amplification product, fluorescence coded microspheres and streptavidin-phycoerythrin; reading the MFI value through a detector; and distinguishing different types of viruses. L'invention concerne un procédé de dosage fluoroimmunologique multiplex pour différencier rapidement le virus de la maladie hémorragique du lapin, le virus Sendai et un rotavirus de lapin, et un réactif. Le procédé de dosage fluoroimmunologique comprend : l'obtention d'un fragment d'amplification cible au moyen d'une PCR; puis l'hybridation d'un produit d'amplification, de microsphères codées par fluorescence et de streptavidine-phycoérythrine; la lecture de la valeur de MFI par l'intermédiaire d'un détecteur; et la différenciation des différents types de v