METHODS OF DETECTING CELL-FREE DNA IN BIOLOGICAL SAMPLES

• Main Authors: LEE, John Richard, CHENG, Alexandre Pellan, DE VLAMINCK, Iwijn, BURNHAM, Philip Smith, SUTHANTHRIAN, Manikkam, DADHANIA, Darshana
• Format: Patent
• Language: English; French
• Published: 11.10.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present disclosure is directed to methods of detecting cell-free DNA (cfDNA) in biological samples and using it to quantify organ damage and identify pathogens. In some aspects, the biological samples are from patients who have undergone solid-organ transplantation. The disclosure is also directed to methods of detecting and analyzing methylation patterns in cell-free DNA from organ transplant patients to identify the presence of pathogens as well as quantify contributing tissue proportions as a measurement of the host response. La présente divulgation concerne des procédés de détection d'ADN acellulaire (ADNcf) dans des échantillons biologiques et leur utilisation pour quantifier des lésions d'organes et identifier des pathogènes. Selon certains aspects, les échantillons biologiques proviennent de patients ayant subi une greffe d'organe solide. Des procédés de détection et d'analyse des motifs de méthylation dans un ADN acellulaire provenant de patients ayant subi une greffe d'organe pour identifier la présence d'agents pathogènes et quantifier des proportions de tissus contribuant à mesurer la réponse de l'hôte sont en outre décrits.