COMPOSITION FOR DIFFERENTIATION OF DENTAL PULP STEM CELLS INTO ODONTOBLAST PROGENITOR CELLS AND IGG OR IGM TYPE MONOCLONAL ANTIBODY SPECIFICALLY BINDING TO SURFACE OF ODONTOBLAST PROGENITOR CELLS

• Main Authors: JANG, Young Joo, KANG, Kyung Jung
• Format: Patent
• Language: English; French; Korean
• Published: 25.10.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a composition for differentiation of dental pulp stem cells into odontoblast progenitor cells and an IgG or IgM type monoclonal antibody specifically binding to the surface of the odontoblast progenitor cells differentiated from the dental pulp stem cells. According to the present invention, when BMP2 and BMP4 are treated in combination at an optimal ratio, the differentiation efficiency of the dental pulp stem cells into the odontoblast progenitor cells is significantly increased, the calcification of a matrix is induced, and the ability of the odontoblast progenitor cells to be differentiated into dentin is enhanced. In addition, when an IgG or IgM monoclonal antibody that specifically binds to the surface of the odontoblast progenitor cells of the present invention is employed, the odontoblast progenitor cells that can be usefully used for tissue regeneration and differentiation can be effectively isolated and purified. La présente invention concerne une composition pour la différenciation de cellules souches de pulpe dentaire en cellules progénitrices des odontoblastes et un anticorps monoclonal de type IgG ou IgM se liant spécifiquement à la surface des cellules progénitrices des odontoblastes différenciées à partir des cellules souches de pulpe dentaire. Selon la présente invention, lorsque BMP2 et BMP4 sont traités en combinaison à un rapport optimal, l'efficacité de différenciation des cellules souches de pulpe dentaire en cellules progénitrices des odontoblastes est significativement accrue, la calcification d'une matrice est induite, et la capacité des cellules progénitrices des odontoblastes à se différencier en dentine est améliorée. De plus, lorsqu'un anticorps monoclonal IgG ou IgM qui se lie spécifiquement à la surface des cellules progénitrices des odontoblastes de la présente invention est utilisé, les cellules progénitrices des odontoblastes qui peuvent être utilisées de manière utile pour la régénération et la différenciation tissulaire peuvent être efficacement isolées et purifiées. 본 발명은 치수줄기세포의 상아 모전구세포로의 분화용 조성물 및 치수 줄기세포에서 분화된 상아 모전구세포의 표면에 특이적으로 결합하는 IgG 또는 IgM 타입 단일 클론 항체에 관한 것이다. 본 발명에 따르면 BMP2와 BMP4를 최적의 비율로 조합하여 처리하는 경우 치수줄기세포의 상아모전구 세포로의 분화 효율이 유의적으로 증가하고 기질의 석회화가 유도되며, 상아모전구 세포의 상아질로의 분화능이 향상된다. 또한 본 발명의 상아모전구 세포 표면에 특이적으로 결합하는 IgG 또는 IgM 단일 클론 항체를 이용하는 경우, 조직 재생 및 분화에 유용하게 사용될 수 있는 상아모전구 세포를 효과적으로 분리, 정제할 수 있다.