IMPROVED CHROMATOGRAPHY RESIN, PRODUCTION AND USE THEREOF

The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-term...

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Bibliographic Details
Main Authors ANDER, Mats, GRANER, Therese, ZOU, Jinyu, OHMAN, Johan, JORSBACK, Anneli
Format Patent
LanguageEnglish
French
Published 24.05.2018
Subjects
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
THEIR RELEVANT APPARATUS
TRANSPORTING
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Summary:The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells. La présente invention se rapporte au domaine de la chromatographie et concerne plus particulièrement la production de résines chromatographiques à affinité vis-à-vis de protéines comprenant des ligands d'affinité sur la base d'un fragment N-terminal d'une intéine divisée, telle qu'un DnaE de Nostoc punctiforme, ainsi que des méthodes d'utilisation associées. Les fragments N-terminaux sont produits dans des corps d'inclusion dans des cellules bactériennes.
Bibliography:Application Number: WO2017EP79101