METHOD FOR CONSTRUCTING CONGENITAL IMMUNODEFICIENCY GENE THERAPY VECTOR AND USE THEREOF

• Main Authors: CHENG, Tianlin, QIU, Zilong
• Format: Patent
• Language: Chinese; English; French
• Published: 22.03.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

Disclosed are a recombinant lentiviral vector expressing the IL2RG gene, and the preparation thereof, wherein the vector is a self-inactivated third-generation lentiviral vector SIN, and the sequence of the IL2RG gene is as shown in SEQ ID NO: 1. Provided are a method for preparing the vector and the use of the vector in the preparation of a drug for treating primary immunodeficiency diseases. L'invention concerne un vecteur lentiviral recombinant exprimant le gène IL2RG et la préparation associée, le vecteur étant un vecteur lentiviral auto-inactivant de type SIN de troisième génération, la séquence du gène IL2RG étant telle que représentée dans la SEQ ID NO : 1. L'invention concerne une méthode de préparation du vecteur et l'utilisation du vecteur dans la préparation d'un médicament servant au traitement de maladies de type immunodéficience primaire. 一种表达IL2RG基因的重组慢病毒载体及其制备。其中，该载体是自身失活的第三代慢病毒载体SIN，所述的IL2RG基因的序列如SEQ ID NO：1所示。提供了该载体的制备方法，以及该载体在制备治疗原发性免疫缺陷病药物中的用途。