PURIFICATION METHOD FOR ANTI-VEGF TYPE MONOCLONAL ANTIBODY

• Main Authors: YANG, Hui, LI, Wenjia, LIN, Xiaoque, MA, Xutong, YANG, Bin
• Format: Patent
• Language: Chinese; English; French
• Published: 15.03.2018
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

A purification method for an anti-VEGF type monoclonal antibody comprises: first, carrying out capture by using a composite material chromatography to separate an antibody from most of components in a harvesting solution; and then, carrying out fine purification by using hydroxyapatite chromatography, so as to further remove host cell pollutants, aggregates and the like, the composite chromatography material being a composite medium under an ion exchange effect and a hydrophobic effect. By means of the method, the content of host cell protein (HCP), DNA, polymers and acidic peaks are reduced, accordingly the objective for remarkably improving the purity of the antibody is achieved; the method is simple to operate and has low lows. La présente invention concerne un procédé de purification pour un anticorps monoclonal de type anti-VEGF comprenant : premièrement, la réalisation d'une capture à l'aide d'une chromatographie en matériau composite pour séparer un anticorps de la plupart des composants dans une solut