METHOD FOR MANUFACTURING THREE-DIMENSIONAL CELL CULTURE SUPPORT HAVING DOUBLE CROSSLINK, AND CASTING TRAY FOR MANUFACTURING THREE-DIMENSIONAL CELL CULTURE SUPPORT

• Main Author: CHA, Mi Sun
• Format: Patent
• Language: English; French; Korean
• Published: 01.02.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention relates to a method for manufacturing a three-dimensional cell culture support having a double crosslink, and a casting tray for manufacturing a three-dimensional cell culture support, wherein the method for manufacturing a three-dimensional cell culture support having a double crosslink is characterized by comprising: a step for preparing a cell mixed hydrogel; a step for manufacturing a casting gel mold in a three-dimensional form; and a step for manufacturing a gelated structure into a three-dimensional form, and the casting tray for manufacturing a three-dimensional cell culture support is characterized by comprising: a tray part provided with a groove and capable of accommodating a gel solution; a mold part that can cover the tray part; and a mold protrusion part provided on the mold part and inserted into the groove when the mold part covers the tray part. La présente invention concerne un procédé de fabrication d'un support tridimensionnel de culture cellulaire présentant une double réticulation et un plateau de moulage pour la fabrication d'un support tridimensionnel de culture cellulaire, le procédé de fabrication d'un support tridimensionnel de culture cellulaire présentant une double réticulation étant caractérisé en ce qu'il comprend : une étape de préparation d'un hydrogel mixte de cellules ; une étape de fabrication d'un moule pour gel de coulée sous une forme tridimensionnelle ; et une étape de fabrication d'une structure gélifiée en une forme tridimensionnelle et le plateau de moulage pour fabriquer un support de culture cellulaire tridimensionnel est caractérisé en ce qu'il comprend : une partie de plateau pourvue d'une rainure et pouvant recevoir une solution de gel ; une partie de moule qui peut recouvrir la partie de plateau ; et une partie de saillie de moule disposée sur la partie de moule et introduite dans la rainure lorsque la partie de moule recouvre la partie de plateau. 본 발명은 이중가교를 갖는 3차원 세포배양 지지체 제조방법 및 3차원 세포배양 지지체 제작을 위한 캐스팅 트레이에 관한 것으로, 이중가교를 갖는 3차원 세포배양 지지체 제조방법은 세포가 혼합된 하이드로젤(cell mixed hydrogel) 제조하는 단계; 3차원 형상의 캐스팅 젤 몰드(casting gel mold)를 제작하는 단계; 및 3차원 형상으로 젤화된 구조체를 제조하는 단계;를 포함하여 이루어지는 것을 특징으로 하며, 3차원 세포배양 지지체 제작을 위한 캐스팅 트레이는 홈이 마련되어, 젤 용액을 수용할 수 있는 트레이부; 상기 트레이부를 덮을 수 있는 몰드부; 상기 몰드부에 마련되며, 상기 몰드부가 상기 트레이부를 덮을 때, 상기 홈에 삽입되는 몰드 돌출부;가 마련되는 것을 특징으로 하는 것이다.