LENTIVIRAL EXPRESSION VECTOR FOR IMPROVING EXPRESSION OF TCTP GENE, AND APPLICATIONS THEREOF

• Main Author: MAO, Kanlang
• Format: Patent
• Language: Chinese; English; French
• Published: 21.12.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A lentiviral expression vector for specifically promoting high expression of a TCTP gene comprises a fundamental sequence, a resistance gene sequence, a multiple-cloning-sites sequence, a promoter sequence, and a TCTP gene cDNA sequence of a pLVX-IRES-puro expression vector. The multiple cloning sites comprise an EcoR I enzyme cutting site and an Spe I enzyme cutting site, the TCTP gene cDNA sequence comprises an EcoR I enzyme cutting site, a TCTP gene coding sequence and an Spe I enzyme cutting site, and the TCTP gene cDNA sequence is forwardly inserted into the multiple-cloning-sites sequence. The lentiviral expression vector can serve as a tool applied to the research and development of drugs related to TCTP. L'invention concerne un vecteur d'expression lentiviral destiné à favoriser spécifiquement l'expression élevée d'un gène TCTP, ledit vecteur comprenant une séquence fondamentale, une séquence de gène de résistance, une séquence de sites multiples de clonage, une séquence de promoteur et une séquence d