BETA-LACTAMASE VARIANTS

The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability, stability in intestinal medium, impr...

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Bibliographic Details
Main Authors DOCQUIER, Jean-Denis, DE GUNZBURG, Jean
Format Patent
LanguageEnglish
French
Published 31.08.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability, stability in intestinal medium, improved activity against one or more antibiotics, improved specific activity and/or improved production in a host cell. La présente invention concerne un polypeptide isolé ayant une activité bêta-lactamase et des séquences d'acide nucléique codant le polypeptide. Le polypeptide isolé selon l'invention est une variante de VIM-2 ayant des propriétés améliorées, par exemple une plus grande stabilité de la protéase, une stabilité en milieu intestinal, une plus grande activité vis-à-vis d'un ou de plusieurs antibiotiques, une plus grande activité spécifique et/ou une production plus importante dans une cellule hôte.
Bibliography:Application Number: WO2017EP53986