ANTIBODY AFFINITY LIGAND WITH INCREASED ALKALI RESISTANCE

• Main Authors: SHIN, Chul Soo, YOO, Young Sun, YOO, Jong Gil, KIM, Ji Eun, HONG, Min Seon, JEON, Ok Seon, KIM, Myoung Hwa, JEONG, Chang Ku, YOO, Jin Hee, JIN, So Ri, KIM, Do Kyun, SONG, Young Ha, KIM, Hyun Kuk, PARK, Je Hyeon
• Format: Patent
• Language: English; French; Korean
• Published: 24.08.2017
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; TRANSPORTING

The present invention relates to an antibody affinity ligand for use in purifying antibodies. In the present invention, it was revealed that an inter-amino acid site between amino acid residues existing at first and second positions toward the N-terminus of "FNK consequent sequence" in A, B, C, or D domain of protein A is vulnerable to alkali and that resistance to alkali can be acquired without the loss of IgG binding capacity by substitution for these amino acids. La présente invention concerne un ligand d'affinité d'anticorps destiné à être utilisé dans la purification d'anticorps. Dans la présente invention, il a été découvert qu'un site inter-acides aminés entre les résidus d'acides aminés existant au niveau des première et seconde positions en direction de la position N-terminale de la « séquence consécutive à FNK » le domaine A, B, C ou D de la protéine A est vulnérable aux alcalis et que la résistance aux alcalis peut être acquises sans perte de la capacité de liaison à l'IgG par substitution de ces acides aminés. 본 발명은 항체의 정제에 사용되는 항체 친화성 리간드에 관한 것으로, 프로테인 A의 A, B, C 또는 D 도메인에서, 'FNK 연속서열'의 N-말단 쪽으로 첫 번째 앞에 존재하는 아미노산과 두 번째 앞에 존재하는 아미노산 사이가 알칼리에 대해 취약성을 보이는 위치임을 구명하고, 이들 아미노산의 치환에 의해 IgG 결합능의 소실 없이 알칼리에 대한 저항성을 획득할 수 있음을 밝혀냈다.