METHOD FOR IDENTIFYING GRAPE SEED EXTRACT AUTHENTICITY USING HPLC FINGERPRINT SPECTRUM

• Main Authors: WANG, Lei, GAO, Wei, LIAN, YunHe, ZHOU, Li, YANG, QingShan
• Format: Patent
• Language: Chinese; English; French
• Published: 06.07.2017
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Disclosed is a method for identifying grape seed extract authenticity using the HPLC fingerprint spectrum, and in particular, a method for identifying the adulteration of a pine bark extract or a peanut skin extract in a grape seed extract. The method comprises the following steps: 1) respectively establishing the HPLC fingerprint spectra of the grape seed extract, the pine bark extract and the peanut skin extract; 2) determining a pine bark extract characteristic peak and a peanut skin extract characteristic peak; 3) subjecting the grape seed extract sample to be tested to liquid chromatography detection, identifying the adulteration of the pine bark extract or the peanut skin extract in the sample to be tested according to whether the chromatogram contains the characteristic peaks of the pine bark extract and/or the peanut skin extract, wherein the addition of more than 3 percent of adulterants can be accurately identified. The method has good stability and reproducibility, high efficiency, obvious identifi