METHOD FOR PRODUCING REDUCING SUGAR FROM LIGNOCELLULOSIC SUBSTRATES

The invention relates to processes for the conversion of biomass into carbohydrates, notably reducing sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in methods wherein cellulose is converted into sugars using a cellulose-converting e...

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Bibliographic Details
Main Authors BIRIKH, Klara, HEIKKILÄ, Matti Wilhelm, SUONPÄÄ, Anu Minna Maaret, MIALON, Antoine Patrice Noël
Format Patent
LanguageEnglish
French
Published 22.06.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to processes for the conversion of biomass into carbohydrates, notably reducing sugars. It provides means and methods for increasing the yield of enzymatic digestion of a biomass, in particular in methods wherein cellulose is converted into sugars using a cellulose-converting enzyme. More in particular, the invention provides a method for producing a reducing sugar from a lignocellulosic material, wherein the lignocellulosic material is contacted with a pectate lyase at a pH between 9 and 12, wherein the pectate lyase comprises an amino acid sequence according to SEQ ID NO: 1 or an amino acid sequence at least 70% identical with SEQ ID NO: 1. L'invention concerne des procédés de conversion de biomasse en glucides, notamment en sucres réducteurs. Elle décrit des moyens et des procédés permettant d'augmenter le rendement de digestion enzymatique d'une biomasse, en particulier dans des procédés dans lesquels la cellulose est convertie en sucres à l'aide d'une enzyme de conversion de cellulose. Plus particulièrement, l'invention concerne un procédé de production d'un sucre réducteur à partir d'un matériau lignocellulosique, le matériau lignocellulosique étant mis en contact avec une pectate lyase à un pH compris entre 9 et 12, la pectate lyase comprenant une séquence d'acides aminés selon SEQ ID NO : 1 ou une séquence d'acides aminés au moins à 70 % identique à SEQ ID NO : 1.
Bibliography:Application Number: WO2016EP80253