TRANSIENT TRANSFECTION METHOD FOR RETROVIRAL PRODUCTION

The invention relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a...

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Bibliographic Details
Main Authors VINK, Conrad, PALLANT, Celeste, VAMVA, Eirini, JOHNSON, Sabine
Format Patent
LanguageEnglish
French
Published 01.06.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a functional substitute thereof. The invention also relates to uses and methods of transient transfection using said nucleic acid vector. L'invention concerne des vecteurs d'acide nucléique ayant une origine de réplication non mammifère et la capacité à contenir au moins 25 kilobases (kb) d'ADN, et est caractérisée en ce que lesdits vecteurs d'acide nucléique comprennent des séquences d'acide nucléique rétrovirales codant : des protéines gag et pol ; et une protéine d'enveloppe ou un substitut fonctionnel correspondant. L'invention concerne également des utilisations et des procédés de transfection transitoire à l'aide dudit vecteur d'acide nucléique.
Bibliography:Application Number: WO2016EP78334