C-KIT MUTATION QUANTITATIVE REAL-TIME PCR ANALYSIS METHOD FOR DIAGNOSING ACUTE MYELOID LEUKEMIA, AND USE THEREOF

• Main Authors: KIM, Myung-Shin, KIM, Yong-Goo, JANG, Woo-Ri
• Format: Patent
• Language: English; French; Korean
• Published: 26.05.2017
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for detecting c-Kit mutations by using a quantitative real-time PCR (qRT-PCR) method in order to diagnose acute myeloid leukemia and, more specifically, to a method for diagnosing acute myeloid leukemia, evaluating the prognosis thereof, and performing a follow-up on minimal residual disease by using a qRT-PCR method for detecting D816V, D816Y, D816H and N822K mutations of a c-KIT gene. La présente invention concerne un procédé de détection de mutations de c-KIT à l'aide d'un procédé quantitatif par PCR en temps réel (qRT-PCR) afin de diagnostiquer une leucémie myéloïde aiguë et, plus particulièrement, un procédé pour le diagnostic d'une leucémie myéloïde aiguë, l'évaluation du pronostic associé et la réalisation d'un suivi d'une maladie résiduelle minimale par l'utilisation d'un procédé qRT-PCR pour détecter les mutations D816V, D816Y, D816H et N822K d'un gène c-KIT. 본 발명은 급성 골수성 백혈병 진단을 위해서 정량 실시간 PCR(qRT-PCR) 방법을 이용하여 ｃ－Ｋｉｔ 돌연변이를 검출하는 방법에 관한 것으로, 보다 구체적으로는 c-KIT 유전자의 D816V, D816Y, D816H, N822K 돌연변이를 검출하는 실시간 정량 PCR 방법에 의해 급성 골수성 백혈병 진다, 예후 평가 및 미세잔존질환의 추적하는 방법에 관한 것이다.