MICROORGANISM PRODUCING LACTIC ACID AND METHOD FOR PRODUCING LACTIC ACID USING SAME

• Main Authors: YANG, Eun Bin, HA, Cheol Woong, KIM, Seon Hye, LEE, Tae Hee, YANG, Young Lyeol, NA, Kyung Su
• Format: Patent
• Language: English; French; Korean
• Published: 15.12.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a Saccharomyces sp. microorganism having a lactic acid productive capacity, and a method for producing a lactic acid using the same. More specifically, the present invention relates to a Saccharomyces sp. microorganism having a lactic acid productive capacity, mutated such that activity of pyruvate decarboxylase (PDC) is weakened or inactivated compared to intrinsic activity, ATP-citrate lyase (ACL) activity is introduced, and a pyruvate biosynthetic pathway is potentiated compared to an intrinsic biosynthetic pathway, and a method for producing a lactic acid using the same. La présente invention concerne un micro-organisme de type Saccharomyces sp. présentant une capacité de production d'acide lactique, et un procédé de production d'un acide lactique à l'aide de celui-ci. Plus particulièrement, la présente invention concerne un micro-organisme de type Saccharomyces sp. présentant une capacité de production d'acide lactique, muté de telle manière que l'activité pyruvate décarboxylase (PDC) est affaiblie ou inactivée par rapport à l'activité intrinsèque, une activité ATP-citrate lyase (ACL) est introduite, et la voie de biosynthèse de pyruvate est potentialisée par rapport à la voie de biosynthèse intrinsèque, ainsi qu'un procédé de production d'un acide lactique à l'aide de celui-ci. 본 발명은 젖산 생산능을 갖는 사카로마이세스 속 (Saccharomyces sp.) 미생물 및 이를 이용한 젖산 제조 방법에 관한 것이다. 보다 구체적으로, 본 발명은 피루베이트 디카르복실라아제 (Pyruvate decarboxylase, PDC)의 활성이 내재적 활성에 비해 약화 또는 불활성화되고, ATP-시트르산 분해효소 (ATP-citrate lyase, ACL) 활성이 도입되고, 피루베이트 생합성 경로가 내재적 생합성 경로에 비하여 강화되도록 변이된, 젖산 생산능을 갖는 사카로마이세스 속 미생물 및 이를 이용한 젖산 제조 방법에 관한 것이다.