NEW DIBASIC ORGANIC ACID PRODUCING STRAIN AND PREPARATION AND APPLICATION OF SAME

• Main Authors: LIU, Qian, LIN, Liangcai, SUN, Tao, XU, Jing, SUN, Wenliang, MA, Yanhe, LI, Jingen, LONG, Chuannan, JI, Jingxiao, TIAN, Chaoguang
• Format: Patent
• Language: Chinese; English; French
• Published: 18.08.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided are an engineering strain for synthesizing a dibasic organic acid and preparation and application of same. The engineering strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid; as compared with the origin strain of the engineering strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate transaminase, glutamic-aspartate transport protein, C4-dicarboxylic transport protein, pyruvate carboxylase, malate dehydrogenase, and glucose transport protein; the expression product of the negative regulator gene comprises succinyl coenzyme A synthase and malic-α-ketoglutaric transport protein; and the origin strain comprises myce