METHOD FOR DETECTING METASTATIC CELLS IN LYMPH NODES OF HEAD AND NECK SQUAMOUS CELL CARCINOMA PATIENTS, AND USE OF MICRORNAS AS MARKERS

• Main Authors: VETTORE, André Luiz, CARVALHO, André Lopes, DE CARVALHO PETERS, Ana Carolina, SCAPULATEMPO NETO, Cristovam
• Format: Patent
• Language: English; French; Portuguese
• Published: 18.08.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention is directed to a test for diagnosing cervical metastasis in head and neck squamous cell carcinoma patients by evaluating the level of expression of the micro-RNAs miR-203 and miR-205. The test can be applied to samples collected using various methods at different points in time, for example during pre-treatment, for diagnosing using fine-needle aspiration biopsy (FNAB), in the intra-operative period through sentinel lymph node biopsy and after surgery, as an auxiliary tool of histopathological evaluation of lymph nodes resected during surgery, assisting in the choice of treatment of such patients from the initial treatment to adjuvant therapy. La présente invention concerne un test pour le diagnostic d'une métastase cervicale chez des patients atteints de CETC par détermination du niveau d'expression des micro-ARN miR-203 et miR-205. Le test peut s'appliquer à des échantillons collectés au moyen de divers abordages à différents moments, notamment en pré-traitement avec le diagnostic au moyen de biopsies de PAAF, en intra-opératoire avec biopsie du lymphonoeud sentinelle et après la chirurgie comme outil auxiliaire pour l'évaluation histopathologique de lymphonoeuds ablatés pendant la chirurgie, facilitant le choix du traitement de ces patients depuis le traitement initial jusqu'à la thérapie adjuvante. A presente invenção tem como objetivo um teste para o diagnóstico de metástase cervical em pacientes com CECP através da avaliação do nível de expressão dos microRNAs miR-203 e miR-205. O teste pode ser aplicado em amostras coletadas utilizando-se várias abordagens em diferentes momentos, como no pré-tratamento através do diagnóstico utilizando biópsias de PAAF, no intra-operatório através de biópsia do linfonodo sentinela e após a cirurgia como uma ferramenta auxiliar para a avaliação histopatológica de linfonodos ressecados durante a cirurgia, ajudando na escolha do tratamento destes pacientes desde o tratamento inicial até a terapia adjuvante.