METHOD AND KIT FOR IDENTIFICATION OF NEMATODES, FOREST PLANT PESTS, BY REAL-TIME PCR

The object of the invention is a method and a kit for identification of nematodes, forest plant pests, by Real-Time PCR, in particular, the invention relates to a method and a kit for identification of nematodes comprising the species Bursaphelenchus mucronatus, Mesocriconema xenoplax and Rotylenchu...

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Main Authors RYCHLICKA, EDYTA, TEREBA, ANNA, ŁOŚ, MARTA, MALEWSKI, TADEUSZ, GRALAK, ALEKSANDRA, RYBARCZYK-MYDŁOWSKA, KATARZYNA, FLIS, ŁUKASZ, ILIEWA-MAKULEC, KRASSIMIRA, KOWALEWSKA, KATARZYNA, WINISZEWSKA-ŚLIPIŃSKA, GRAŻYNA, POMORSKI, JAN JAKUB, TURLEJ, ROBERT, WIŚNIEWSKA, KATARZYNA, SKWIERCZ, ANDRZEJ, DMOWSKA, EWA, BOGDANOWICZ, WIESŁAW, TOMALAK, MAREK, WIŚNIEWSKA, OLGA
Format Patent
LanguageEnglish
French
Published 21.07.2016
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The object of the invention is a method and a kit for identification of nematodes, forest plant pests, by Real-Time PCR, in particular, the invention relates to a method and a kit for identification of nematodes comprising the species Bursaphelenchus mucronatus, Mesocriconema xenoplax and Rotylenchus uniformis. Due to the specifically selected primers and probes the solution according to the invention enables accurate identification of the selected nematode species regardless of the analyzed sample type, their origin and purpose, as well as developmental stage. L'invention porte sur un procédé et un kit pour l'identification, par PCR en temps réel, de nématodes nuisibles envers les végétaux des milieux forestiers. En particulier, l'invention concerne un procédé et un kit pour l'identification de nématodes comprenant les espèces Bursaphelenchus mucronatus, Mesocriconema xenoplax et Rotylenchus uniformis. Les amorces et les sondes sélectionnées spécifiquement de la solution de l'invention permettent d'identifier avec exactitude l'espèce de nématode sélectionnée, indépendamment du type d'échantillon analysé, de son origine et de ses fins, ainsi que stade de développement.
Bibliography:Application Number: WO2015PL00092