METHOD FOR REGULATING GENE EXPRESSION USING CAS9 PROTEIN EXPRESSED FROM TWO VECTORS

• Main Authors: KIM, JIN SOO, KOO, TAE YOUNG
• Format: Patent
• Language: English; French; Korean
• Published: 26.05.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for regulating a gene comprising a step of introducing, into a cell, a recombinant vector expressing a first domain including the N-terminal of a Cas9 protein and a recombinant vector expressing a second domain including the C-terminal of the Cas9 protein; a composition comprising the recombinant vectors; a gene expression regulation kit; and a method for intracellular preparation of a Cas9 protein. In addition, the present invention relates to a composition comprising a transformed cell, into which a virus vector packaging the first domain and a virus vector packaging the second domain are introduced, and a virus produced therefrom. La présente invention concerne un procédé pour réguler un gène comprenant une étape consistant à introduire, dans une cellule, un vecteur recombinant exprimant un premier domaine comprenant l'extrémité N d'une protéine Cas9 et un vecteur recombinant exprimant un deuxième domaine comprenant l'extrémité C de la protéine Cas9 ; une composition comprenant les vecteurs recombinants ; un kit de régulation d'expression génique ; et un procédé de préparation intracellulaire d'une protéine Cas9. En outre, la présente invention concerne une composition comprenant une cellule transformée, dans laquelle un vecteur de virus encapsidant le premier domaine et un vecteur de virus encapsidant le deuxième domaine sont introduits et un virus produit à partir de ceux-ci. 본 발명은 Cas9 단백질의 N-말단을 포함하는 제1 도메인을 발현하는 재조합 벡터 및 C-말단을 포함하는 제2 도메인을 발현하는 재조합 벡터를 각각 세포 내에 도입하는 단계를 포함하는, 유전자 조절 방법, 상기 재조합 벡터를 포함하는 조성물, 유전자 발현 조절용 키트, 및 Cas9 단백질의 세포 내 제조 방법에 관한 것이다. 또한, 본 발명은 상기 제1 도메인을 패키징하는 바이러스 벡터 및 제2 도메인을 패키징하는 바이러스 벡터가 도입된 형질전환 세포 및 이로부터 생산된 바이러스를 포함하는 조성물에 관한 것이다.