LINKER ELEMENT AND METHOD OF USING SAME TO CONSTRUCT SEQUENCING LIBRARY

• Main Authors: DRMANAC, RADOJE, ZHAO, XIA, WU, FANZI, JIANG, HUI, FU, SHUJIN, ZHANG, WENWEI, ALEXEEV, ANDREI, SU, XIAOSHAN, LI, YAQIAO, JIANG, YUAN, GENG, CHUNYU, HE, LINGYU
• Format: Patent
• Language: Chinese; English; French
• Published: 21.04.2016
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMBINATORIAL CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3' end of the short strand has an enclosed modification, with enzyme sites in the short strand; the linker B is a nucleic acid single strand, the 3' end thereof can be in a complementary pairing with the 5' end of the long strand of the linker A; and there are II type restriction endonuclease recognition sites in the long strand of the linker A and the linker B. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps,