METHOD AND MEASURING DEVICE FOR MEASURING CONCENTRATION OF ANALYTE IN BIOLOGICAL SAMPLE

• Main Authors: CHOI, SUNG HYUK, NAM, HAKHYUN, LEE, SEOK-WON, LEE, MYEONGHO, CHO, SUNG PIL, CHA, GEUN SIG, KANG, YOUNGJEA, PARK, HO DONG
• Format: Patent
• Language: English; French; Korean
• Published: 24.03.2016
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A method for measuring the concentration of an analyte in a biological sample by using an electrochemical biosensor, according to one embodiment of the present invention, with respect to chronoamperometry by which, after injecting a whole blood sample into the electrochemical biosensor, the concentration of an analyte is measured from a faradic current obtained by applying a direct current voltage for a certain time, comprises the step of obtaining predetermined features from the total faradic currents obtained from the faradic current value obtained by applying the direct current voltage, and from the additional application of a plurality of step-ladder perturbation potentials during a short time following the application of the direct current voltage for a certain time, and the method creates a calibration equation by combining at least one or more of the features by a function, and optimizes the features with respect to the various conditions of the biological sample by means of multivariable analysis, thereby minimizing error in measurement due to interfering material. L'invention concerne un procédé destiné à mesurer la concentration d'un analyte dans un échantillon biologique au moyen d'un biocapteur électrochimique. Dans un mode de réalisation de l'invention, par rapport à une chronoampérométrie qui, après injection d'un échantillon de sang total dans le biocapteur électrochimique, permet de mesurer la concentration d'un analyte à partir d'un courant faradique obtenu par application d'une tension de courant continu pendant un certain temps, le procédé selon l'invention consiste à obtenir des caractéristiques prédéterminées à partir de la totalité des courants faradiques obtenus à partir de la valeur de courant faradique obtenue par application de la tension de courant continu, et à partir de l'application supplémentaire d'une pluralité de potentiels de perturbation en escabeau pendant un court laps de temps après l'application de la tension de courant continu pendant un certain temps; et à créer une équation d'étalonnage par combinaison d'au moins une des caractéristiques au moyen d'une fonction, et à optimiser les caractéristiques par rapport aux diverses conditions de l'échantillon biologique au moyen d'une analyse multi-variables, ce qui permet de réduire au minimum les erreurs de mesure dues à un matériau perturbateur. 본 발명의 일 실시예에 따른 전기화학적 바이오센서를 이용한 생체시료내 분석대상물질의 농도측정방법은, 상기 전기화학적 바이오센서에 전혈시료를 주입한 후, 일정 시간 직류전압을 인가하여 얻은 감응전류로부터 분석대상물질(analyte)의 농도를 구하는 대시간전류법에 대하여, 직류전압을 인가하여 얻게 되는 감응전류 값과 상기 일정시간 직류전압에 이어서 짧은 시간 동안 수 개의 계단화된 사다리형 섭동전압(step-ladder perturbation potential)을 더 인가하여 얻게 되는 전체 감응전류들에서 미리 정해진 피쳐(feature)들을 얻는 단계와, 상기 적어도 1 이상의 피쳐를 함수로 결합하여 검정식(calibration equation)을 만들고, 상기 생체시료의 다양한 조건들에 대하여 다변수회귀분석(multivariable analysis)으로 최적화함으로써 방해물질에 의한 측정의 오차를 최소화시키는 것을 특징으로 한다.