NEW ANTI-HUMAN PAI-1 ANTIBODY

• Main Authors: TANAKA, HIROTSUGU, YOSHINO, MASAYASU
• Format: Patent
• Language: English; French; Japanese
• Published: 27.08.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

[Problem] To provide an anti-human PAI-1 antibody for prevention and treatment of pulmonary fibrosis by binding to active human PAI-1 and inhibiting effects mediated by active human PAI-1. [Solution] The inventors studied anti-PAI-1 antibodies, and provided an anti-human PAI-1 antibody that contains: a heavy-chain variable region comprising the amino acid sequence from amino acid 1 to 118 in SEQ ID No. 2; and a light-chain variable region comprising the amino acid sequence from amino acid 1 to 108 in SEQ ID No. 4. L'invention concerne un nouvel anticorps anti-PAI-1 humain pour la prévention et le traitement de la fibrose pulmonaire, par liaison avec un PAI-1 humain actif et inhibition des effets produits par ce PAI-1 humain actif. Plus spécifiquement, l'étude des anticorps anti-PAI-1 a permis d'obtenir un anticorps anti- PAI-1 humain contenant: une région variable à chaînes lourdes constituée de séquences d'amino-acides 1 à 118 dans SEQ ID no2, ainsi qu'une région variable à chaînes légères constituée de séquences d'amino-acides4 à 108 dans SEQ ID no4. 【課題】　活性型ヒトＰＡＩ－１に結合して、活性型ヒトＰＡＩ－１を介する作用を阻害することにより肺線維症を予防又は治療する抗ヒトＰＡＩ－１抗体を提供することにある。 【解決手段】　本発明者らは、抗ＰＡＩ－１抗体について検討し、配列番号２のアミノ酸番号１から１１８までのアミノ酸配列からなる重鎖可変領域、及び配列番号４のアミノ酸番号１から１０８までのアミノ酸配列からなる軽鎖可変領域を含む、抗ヒトＰＡＩ－１抗体を提供した。