RECOMBINANT L-ASPARAGINASE FROM ZYMOMONAS

• Main Authors: LARENTIS, ARIANE LEITES, MOITINHO ALVES, TITO LÍVIO, EISNFELDT, KAREN, CAVALCANTE BAPTISTA, ISIS, SOBRAL DA COSTA, ELAINE, MENKS RIBEIRO, MARIA CECÍLIA, VOLCAN ALMEIDA, RODRIGO, POIROT LAND, MARCELO GRERADIN
• Format: Patent
• Language: English; French; Portuguese
• Published: 16.07.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The present invention relates to the construction and optimization of synthetic genes from the Zymomonas mobilis L-asparaginase gene, the method for cloning same and expression thereof in Escherichia coli. The purpose of the production of said enzyme is for producing high levels of a novel L-asparaginase that can be used in L-asparaginase-based pharmaceutical compositions for treating cancer, tumours and diseases involving cell proliferation, as well as for other medical applications. La présente invention concerne la construction et l'optimisation de gènes synthétiques à partir du gène de L-asparaginase de Zymomonas mobilis, son clonage et son expression dans Escherichia coli. L'obtention de cette enzyme a pour objectif de produire de hauts niveaux d'une nouvelle L-asparaginase en vue d'une utilisation dans des compositions pharmaceutiques à base de L-asparaginase à utiliser dans le traitement du cancer, de tumeurs, de maladies impliquant une prolifération cellulaire, ainsi que dans d'autres applications médicales. A presente invenção relata a construção e otimização de genes sintéticos a partir do gene de L-asparaginase de Zymomonas mobilis, sua clonagem e expressão em Escherichia coli. A obtenção de tal enzima tem por objetivo produzir altos níveis de uma nova L-asparaginase para uso em composições farmacêuticas a base de L-asparaginase a serem utilizadas no tratamento de câncer, tumores, doenças com proliferação celular, assim como outras aplicações médicas.