OVER-EXPRESSION OF A FATTY ACID TRANSPORTER GENE AND OF GENES ENCODING ENZYMES OF THE BETA-OXIDATION PATHWAY FOR HIGHER PRODUCTION OF RIBOFLAVIN VIA FERMENTATION OF EREMOTHECIUM

• Main Authors: ZELDER, OSKAR, MOLT, ANDREA, HOFF, BIRGIT, HAEFNER, STEFAN
• Format: Patent
• Language: English; French
• Published: 18.06.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The present invention relates to a method of producing riboflavin in a genetically modified organism of the genus Eremothecium, wherein said genetic modification is linked to the fatty acid uptake and/or beta-oxidation pathway of said organism, comprising growing said organisms in a culture medium and isolating riboflavin from the culture medium. The invention further relates to a method of providing a riboflavin accumulating organism belonging to the genus Eremothecium by genetically modifying said organism, to organisms obtained by such a method, as well as the use of such genetically modified organisms for increasing the accumulation of riboflavin. La présente invention concerne un procédé de production de riboflavine dans un organisme génétiquement modifié du genre Eremothecium, ladite modification génétique étant liée à l'absorption des acides gras et/ou la voie de bêta-oxydation dudit organisme, comprenant la croissance desdits organismes dans un milieu de culture et l'isolement de riboflavine à partir du milieu de culture. L'invention concerne en outre un procédé d'obtention d'un organisme accumulant la riboflavine appartenant au genre Eremothecium par modification génétique dudit organisme, les organismes obtenus par ce procédé, ainsi que l'utilisation de ces organismes génétiquement modifiés pour augmenter l'accumulation de riboflavine.