SYSTEMS AND METHODS FOR UNIVERSAL TAIL-BASED INDEXING STRATEGIES FOR AMPLICON SEQUENCING

Some embodiments of the invention include a method of preparing a sample for sequencing that includes receiving a sample and amplifying at least one marker within the sample. In some embodiments, amplification of the first marker may include mixing the sample with a first oligonucleotide that compri...

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Bibliographic Details
Main Authors ENGELTHALER, DAVID, SCHUPP, JAMES M, HICKS, NATHAN, KEIM, PAUL S, COLMAN, REBECCA, GILLECE, JOHN
Format Patent
LanguageEnglish
French
Published 14.05.2015
Subjects
DIAGNOSIS
HUMAN NECESSITIES
HYGIENE
IDENTIFICATION
MEDICAL OR VETERINARY SCIENCE
SURGERY
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Summary:Some embodiments of the invention include a method of preparing a sample for sequencing that includes receiving a sample and amplifying at least one marker within the sample. In some embodiments, amplification of the first marker may include mixing the sample with a first oligonucleotide that comprises a first universal tail sequence and a second oligonucleotide that comprises a second universal tail sequence. In some aspects of the invention, the first universal tail sequence and the second universal tail sequence are different sequences. La présente invention concerne, selon certains modes de réalisation, un procédé de préparation d'un échantillon en vue de son séquençage comprenant les étapes consistant à recevoir un échantillon et à amplifier au moins un marqueur présent dans l'échantillon. Selon certains modes de réalisation, l'amplification du premier marqueur peut comprendre le mélange de l'échantillon avec un premier oligonucléotide comportant une première séquence de queue universelle et un second oligonucléotide comportant une seconde séquence de queue universelle. Selon certains aspects de l'invention, les première et seconde séquences de queue universelles correspondent à des séquences différentes.
Bibliography:Application Number: WO2014US64890