METHOD FOR ISOLATION, PURIFICATION AND INDUSTRIAL SCALE EXPANSION OF HUMAN ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS

• Main Authors: SODHI, MINITA, LAGHATE, SNEHA DEEPAK, GOWDA, SHASHANK, CHOUGULE, BASAVARAJ, HARI, AARYA, FONSECA, LYLE CARL, TOTEY, SATISH MAHADEORAO, REDDY, MANOJ KUMAR C, KOSHY, NICOLE RACHEL
• Format: Patent
• Language: English; French
• Published: 26.03.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to a method for isolation, purification and industrial scale expansion of human adipose tissue derived mesenchymal stem cells (MSCs). The invention also relates to a method for treating and a therapeutic product for treating type-1 diabetes mellitus, critical limb ischemia, multiple sclerosis, Duchenne muscular dystrophy, rheumatoid arthritis, cerebral stroke, type-II diabetes, idiopathic pulmonary fibrosis, dilated cardiomyopathy, and osteoarthritis comprising MSCs. La présente invention concerne un procédé pour l'isolement, la purification et la mise à l'échelle industrielle de cellules souches mésenchymateuses (CSM) dérivées du tissu adipeux humain. L'invention concerne également un procédé de traitement et un produit thérapeutique pour traiter le diabète de type 1, l'ischémie critique des membres, la sclérose en plaques, la dystrophie musculaire de Duchenne, la polyarthrite rhumatoïde, l'AVC, le diabète de type II, la fibrose pulmonaire idiopathique, la cardiomyopathie dilatée et l'arthrose comprenant des CSM.