FLAVOBACTERIUM STRAIN, ENDO-ALGINATE LYASE ENCODING GENE, AND PREPARATION AND USE OF SAID GENE

• Main Authors: HUANG, LISHUXIN, CAO, HAILONG, DU, YUGUANG, ZHAO, XIAOMING, LIU, HANG, LI, SHUGUANG
• Format: Patent
• Language: Chinese; English; French
• Published: 20.06.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

Provided is a gene sequence for endo-alginate lyase Alg2A derived from Flavobacterium sp. strain S20 freshly isolated from soil samples, and a preparation method and application therefor. Also provided is a method of preparing the alginate lyase Alg2A, i.e. a technical method utilizing genetic engineering, in which the Alg2A gene is cloned to an Escherichia coli expression vector to obtain an Escherichia coli recombinant strain that can heterologously express said lyase. Using said strain to heterologously express the prepared alginate lyase Alg2A has the function of decomposing sodium alginate and preparing sodium alginate oligosaccharide. The present invention applies to the chemical, agricultural, and food industries, as well as to livestock feed processing, to the field of medicine, and to the field of genetic engineering of marine algae. La présente invention se rapporte à une séquence de gène pour une lyase d'endo-alginate Alg2A dérivée d'une souche de l'espèce Flavobacterium S20 fraîchement isolée à pa