KETOL-ACID REDUCTOISOMERASES WITH IMPROVED PERFORMANCE PROPERTIES

• Main Authors: SNOW, CHRISTOPHER, MEINHOLD, PETER, SMITH, CHRISTOPHER, PORTER-SCHEINMAN, STEPHANIE, BERRY, RUTH, BUELTER, THOMAS, LIES, DOUGLAS
• Format: Patent
• Language: English; French
• Published: 04.04.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGYGENERATION, TRANSMISSION OR DISTRIBUTION; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol. La présente invention concerne des microorganismes recombinants comprenant au moins une molécule d'acide nucléique codant pour une réductoisomérase cétol-acide (KARI) ou un variant modifié dépendant de NADH de celle-ci, ladite KARI étant au moins environ à 60 % identique à SEQ ID No:2. Dans divers aspects de l'invention, les microorganismes recombinants peuvent comprendre une voie métabolique de production d'isobutanol et peuvent être utilisés dans des procédés de fabrication d'isobutanol.